Human milk oligosaccharides (HMOs) perform a number of functions including serving as prebiotics to stimulate the growth of beneficial intestinal bacteria, as receptor analogues to inhibit binding of pathogens, and as substances that promote postnatal brain development.
Sodium ion did not have high affinity to the 2-aminopyridine part comparing to the normal reducing end residue. be neglected for ionization and fragmentation to them. . endobj Proprionyl chloride: -COCl (carbonyl, chlorine). In contrast, in LID MALDI MS of [M + Na] + for 3-NHAc-β-CD, HexNAc-containing fragments are at least twice more abundant than corresponding [Glc n + Na] + ions (Figure 9).
Acetamide is an organic compound with the formula CH3CONH2. >> Use of nitrate and other anionic adducts for the production of negative ion electrospray spectra from, Carbohydrate molecular weight profiling, sequence, linkage, and branching data: ES-MS and CID, Postsource decay fragment spectra of cyclomalto-octaose and branched cyclomaltohexaose by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, Ion intensity analysis of post-source decay fragmentation in curved-field reflectron matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of carbohydrates: For structural characterization of glycosylation in proteome analysis, Tandem mass spectra of glycan substructures enable the multistage mass spectrometric identification of determinants on oligosaccharides, Development of an annotated library of neutral human milk oligosaccharides, Employment of tandem mass spectrometry for the accurate and specific identification of oligosaccharide structures, Distinguishing of linkage isomers of lactotetra oligosaccharides by using the relative ion intensity analysis of post-source decay fragment ions in curved-field reflectron matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, An automated interpretation of MALDI/TOF postsource decay spectra of oligosaccharides. An analysis of the relative ion intensities in the MALDI-PSD mass spectra of oligosaccharides was very useful for distinguishing the linkage isomers and for characterizing the types of glycosyl linkages. In ES-CID-MS, the carbohydrate chemist has a powerful new tool in hand for structural elucidations that can be conducted at the low-picomole level. ?��?\��1s �^�����ǁ��x.Ά�����t�H���P���krۚn]��_�/��ß.� ������͗�p�GPh����_�5�Tr4rR;����u���#�_SK��. HPLC-Chip/TOF MS provides a sensitive and quantitative method for sample profiling. >> not otherwise permitted to reproduce, republish, redistribute, or sell any Supporting Information
CyD derivatives substituted by amino or acetamide groups are ideal analytes to extract the function group effects, which are amino-CyD with one hexosamine (HexNH2) and acetamide-CyD with one N-acetyl hexosamine (HexNAc). The various kinds of oligosaccharides were analyzed by using post-source decay (PSD) fragmentation method of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. The peaks labeled by blue and red arrows originated from the chemical species with or without, MALDI LID MS/MS product ion spectrum of malto-tetraose, in the protonated molecules easily, and the small product, ions are generated abundantly. /Annots [86 0 R 87 0 R 88 0 R] /Rotate 0 /Thumb 54 0 R The ion peaks labeled with blue arrows are assigned to, peaks labeled with red arrows are assigned to the species, ion intensity of the product ion with HexNAc is much, sodium cations localize on the acetamide hexose residue, The MALDI LID product ion spectra of tetrasaccharides, compared to generally prove the acetamide group effects on, product ions with a reducing terminal have a higher intensity, than the B type ions in the spectrum of a homogeneous, glucotetraose of maltotetraose. This section summarizes several strategies for a more complete understanding of carbohydrate structure with a focus on glycolipids and glycoprotein glycans. J. Proteome Res. Fraser-Reid and coworkers have investigated this group as an alternative for the acetamido group (Scheme 10).