German botanist G. Haberlandt is regarded as the father of tissue culture. Basic Technique of Plant Tissue Culture: The general procedure adopted for isolation and culture of plant tissues is depicted in Fig. The minimum times required for sterilization of different volumes of medium are listed below. For this method, explants (tissue from the plant) are obtained from an in-vitro derived plant and introduced in to an artificial environment, where they get to proliferate. In most cases the microorganisms outgrow the plant tissues, resulting in their death. Contamination can also spread from culture to culture. Sterilization procedures involve the use of heat, radiation, chemicals or physical removal of Abdi (2008) observed an optimum result for explant disinfection by using 120 mg l-1 of nano-silver solution after surface sterilization of valerian tissue culture, without any deleterious effect on explants. Cleaning: Cleaning is removal of visible soil (e.g., organic and inorganic material) from objects and surfaces. In this way, the micropropagation is the only method that reliable the production of commercial clonal plantlets without phytosanitary problems (CARDOSO and TEIXEIRA DA SILVA, 2013). The recommended dose is 0.5–2.0 mL of PPM™ per liter of medium. In a cell culture technique, cells are removed from an animal or a plant and grown subsequently in a favorable environment. The electrical energy is mainly used in plant tissue culture labs for control temperature and light in growth Rostami and Shahsavar (2009) reported that adding nano-silver with concentration of 4 Disinfectants of the phenol group—lysol and cresol are powerful disinfectants for dis­infecting surgical instruments and discarded … There are many types of filtration techniques, but when sterilizing a system membrane filtration is used. So of the available methods, Methods of sterilization of surgical instruments are Boiling, Incineration, and Autoclave. materials in the plant tissue culture. Method # 7. The parameters which have been determined to have a significant effect on the growth of in vitro propagated Eucheuma seaweeds are the sterilization techniques, media composition, light intensity and aeration. The most important of these is the plant itself, and plant material should be well sterilized before being isolated in vitro. Plant tissue culture media are generally sterilized by autoclaving at 121 °C and 1.05 kg/cm 2 (15-20 psi). The sterilized materials are removed when temperature cools down and used according to procedure adopted. ... 10 Micro-propagation of plant By shoot tip & nodal culture method. Effective Sterilization Protocol for Micropropagation of Musa coccinea (Musa SPP) Reza Farzinebrahimi 1 , Kamaludin Rashid 2, Rosna Mat Taha 1, Jamilah Syafawati Yaacob 1 1 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Malaysia Centre for 2 Foundation Studies of Science, University of Malaya, Kuala Lumpur, 50603, Malaysia Share yours for free! - Basic techniques of cell and tissue culture, surface sterilization, aseptic tissue transfer, concept of totipotency. 41 Sterilisation and Disinfection MICROBIOLOGY MODULE Microbiology Notes Disinfection: Disinfection describes a process that eliminates many or all pathogenic microorganisms, except bacterial spores, on inanimate objects. Sterilization of Glassware, Equipments and Culture Media The thoroughly washed glassware and equipments and carefully prepared culture media are sterilized by heat, steam or millipore filter paper (Table 6.4 ). 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