Learn about our remote access options, Institute of Chemistry, National Taiwan Normal University, Taipei 117, Department of Chemistry, Providence College of Arts and Sciences, Taichung, Taiwan 400. The clove oil, round bottom flask, and cork base were weighed at 105. The separatory funnel was capped and inverted allowing the solution to mix and the gas was released from the top.
4 % recovery from the ground cloves, the IR was analyzed. By continuing we’ll assume you’re on board with our cookie policy, The input space is limited by 250 symbols. Conclusion After obtaining clove oil through steam distillation, the IR was successful in identifying the major constituent as eugenol.
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The deformation of macromolecules in the membrane, upon treatment with eugenol was verified by FT-IR spectroscopy. Although a low percent recovery was obtained the sample of clove oil was pure, which further proves that the major constituent can be identified as eugenol. The bottom layer of the separatory funnel was added to a 250 mL beaker. The bottom layer of the separatory funnel was added to the same 50 mL flask.
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The Bunsen burner was lit and then used to “paint” the bottom of the 500 mL round bottom flask with water and ground cloves in it. Eugenol will be extracted from the distillate with dichloromethane and analyzed using the Thermo Scientific picoSpin 45 or Thermo Scientific picoSpin 80 NMR spectrometers.
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1. The purpose of this lab was to obtain a pure sample of clove oil from ground cloves using steam distillation, perform infrared spectroscopy on the sample, and analyze the IR to identify the major constituent in the clove oil. Isolation of the Major Constituents of Clove Oil. A 100 mL round bottom flask and cork base were weighed at 104. b. Clove Oil Characterization #is experiment used clove oil taken from MITRA PALA MAS, Kampung Krajan Wanayasa, Purwakarta. endobj
The IR of the experimentally obtained clove oil (Figure 1) was then compared to the known IR of eugenol (Figure 2), which was relatively similar except for the short wide O-H peak in figure 1 and the medium thin O-H peak in figure 2. After the clove oil was isolated it was analyzed using IR spectroscopy. 356 g. Magnesium sulfate was added to the beaker until the powder stopped clumping together. The following list only include the common oil names without important details. The role of IR spectrum analysis Both the basic additives in engine oils and the acid build up in other types of fluids can be seen as changes in the infrared spectrum of a sample. 5.
0.3%) and from 13C‐NMR method is 90.5% (s.d. Access a targeted collection of application notes, case studies, videos, webinars and white papers covering a range of applications for Fourier Transform infrared spectroscopy, Near-infrared spectroscopy, Raman spectroscopy, Nuclear Magnetic Resonance, Ultraviolet-Visible (UV-Vis) spectrophotometry, X-Ray Fluorescence, and more. Calculations The weight of clove oil obtained was found using the following equation. The flask was then labeled sealed using a cork stopper and parafilm. Preliminary studies on the mid-infrared analysis of edible . 50. The mode of action of eugenol was determined by crystal violet assay, measurement of release of 260 nm absorbing material, SDS-PAGE, FT-IR spectroscopy, AFM and SEM. The first day the clove oil was removed from the ground cloves into an aqueous solution then removed from the water using DCM.